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| Brum,Karine Bonucielli; Haraguchi,Mitsue; Garutti,Mirella Biasoli; Nóbrega,Fernanda Nogarol; Rosa,Beneval; Fioravanti,Maria Clorinda Soares. |
| Brachiaria species contain steroidal saponins and are involved in outbreaks of hepatogenous photosensitization. This research presents the levels of a steroidal saponin, protodioscin, in the seeds and aerial parts of B. brizantha and B. decumbens during different developmental stages (growth, bloom, fructification and seed fall). The butanolic fraction of the ethanolic extract of each stage was submitted to thin layer chromatography (TLC) and spectrophotometric analysis through the Ehrlich reagent in 515nm. The chromatograms in TLC of the butanolic fraction of B. brizantha and B. decumbens showed similar spots as the protodioscin standard. The estimated level of protodioscin isomers in B. brizantha and B. decumbens ranged from 0.5% to 2.1%, having the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brachiaria spp.; Photosensitization; Protodioscin; Spectrophotometry; Steroidal saponins. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000100049 |
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| GAWADE,S. P.. |
| The photodynamic action of UV sensitized methylene blue (MB) was studied on the venom of Thailand cobra Naja siamensis. The reaction mixture comprising phosphate buffer (B), methylene blue (S), and venom protein (P) was exposed to UVR (ultra violet radiation) for 2 hours at 37°C. Stable excited species (S) were generated by photodynamic action. These in B environment produced reduced substates SH2 and photooxidised products PO in the presence of atmospheric oxygen. Increased absorbance (OD) of the reaction mixture after UVR with a corresponding loss of protein was suggested to be due to the formation of photooxidised venom products. The detoxification profile of venom protein using visible radiation in the presence of MB and oxygen appeared similar to the... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Thailand cobra; Naja siamensis; Photosensitization; Photooxidation; Detoxification. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302000000200010 |
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